LDV Vans Trophy 2000-01 Winners: Port Vale Runners-up: Brentford Winners from previous season: Stoke City First round
| Northern section | Blackpool, Macclesfield Town, Shrewsbury Town and Walsall given byes to the second round. | 28 November 2000 | Lincoln City | 3 | - | 2 | Morecambe | 1,194 | Sincil Bank, Lincoln | 5 December 2000 | Chester City | 1 | - | 0 | Hull City | 770 | Deva Stadium, Chester | 5 December 2000 | Doncaster Rovers (won in sudden death extra time by golden goal) | 3 | - | 2 | Rochdale | 1,453 | Belle Vue, Doncaster (aet) | 5 December 2000 | Rotherham United | 3 | - | 4 | Chesterfield (won in sudden death extra time by golden goal) | 3,488 | Millmoor, Rotherham (aet) | 6 December 2000 | Stoke City | 3 | - | 1 | Scarborough | 2,336 | Victoria Ground, Stoke | 9 December 2000 | Bury | 2 | - | 1 | Mansfield Town | 1,117 | Gigg Lane, Bury | 9 December 2000 | Wrexham | 0 | - | 1 | Halifax Town | 1,545 | Racecourse Ground, Wrexham | 11 December 2000 | Hartlepool United | 3 | - | 2 | Scunthorpe United | 1,538 | Victoria Park, Hartlepool | 12 December 2000 | Kidderminster Harriers (won in sudden death extra time by golden goal) | 2 | - | 1 | Carlisle United | 777 | Aggborough, Kidderminster (aet) | 9 January 2001 | Oldham Athletic | 2 | - | 3 | Wigan Athletic | 2,551 | Boundary Park, Oldham (aet) | 9 January 2001 | Port Vale | 3 | - | 0 | Notts County | 1,919 | Vale Park, Burslem | 9 January 2001 | York City | 0 | - | 4 | Darlington | 1,095 | Bootham Crescent, York | Southern Section
| Leyton Orient, Reading, Swansea City and Swindon Town given byes to the second round. | 28 November 2000 | Barnet | 2 | - | 0 | Rushden & Diamonds | 887 | Underhill, Barnet | 5 December 2000 | AFC Bournemouth (won 4-2 on pens.) | 1 | - | 1 | Dover Athletic | 2,171 | Dean Court, Bournemouth (aet) | 5 December 2000 | Brentford | 4 | - | 1 | Oxford United | 1,517 | Griffin Park, London | 5 December 2000 | Brighton & Hove Albion | 2 | - | 0 | Cardiff City | 2,364 | Withdean Stadium, Brighton | 5 December 2000 | Cambridge United | 2 | - | 0 | Colchester United | 1,555 | Abbey Stadium, Cambridge | 5 December 2000 | Millwall | 4 | - | 1 | Northampton Town | 2,369 | The New Den, London | 5 December 2000 | Peterborough United | 1 | - | 0 | Luton Town | 2,075 | London Road, Peterborough | 5 December 2000 | Plymouth Argyle | 3 | - | 0 | Bristol City | 1,364 | Home Park, Plymouth | 5 December 2000 | Southend United | 2 | - | 0 | Cheltenham Town | 1,000 | Roots Hall, Southend-on-Sea | 5 December 2000 | Wycombe Wanderers | 1 | - | 0 | Exeter City | 1,022 | Adams Park, High Wycombe | 9 December 2000 | Torquay United | 0 | - | 2 | Bristol Rovers | 1,370 | Plainmoor, Torquay | 19 December 2000 | Hereford United | 4 | - | 0 | Yeovil Town | 853 | Edgar Street, Hereford | Second round | Northern section | 9 January 2001 | Chesterfield | 4 | - | 2 | Macclesfield Town | 1,839 | Recreation Ground, Chesterfield | 9 January 2001 | Halifax Town | 2 | - | 3 | Stoke City | 1,917 | The Shay, Halifax | 9 January 2001 | Hartlepool United | 3 | - | 1 | Doncaster Rovers | 2,466 | Victoria Park, Hartlepool | 9 January 2001 | Lincoln City | 3 | - | 1 | Blackpool | 962 | Sincil Bank, Lincoln | 10 January 2001 | Bury | 2 | - | 0 | Kidderminster Harriers | 1,015 | Gigg Lane, Bury | 16 January 2001 | Darlington | 2 | - | 0 | Shrewsbury Town | 1,502 | Feethams, Darlington | 16 January 2001 | Port Vale | 2 | - | 0 | Chester City | 2,507 | Vale Park, Burslem | 30 January 2001 | Walsall | 2 | - | 1 | Wigan Athletic | 3,436 | Bescot Stadium, Walsall | Southern section | 9 January 2001 | AFC Bournemouth | 0 | - | 1 | Swansea City | 3,810 | Dean Court, Bournemouth | 9 January 2001 | Brighton & Hove Albion | 2 | - | 2 | Brentford (won 4-2 on pens.) | 2,482 | Withdean Stadium, Brighton (aet) | 9 January 2001 | Bristol Rovers | 3 | - | 0 | Plymouth Argyle | 3,781 | Memorial Stadium, Bristol | 9 January 2001 | Hereford United | 1 | - | 2 | Reading | 1,693 | Edgar Street, Hereford | 9 January 2001 | Leyton Orient | 0 | - | 2 | Wycombe Wanderers | 946 | Brisbane Road, London | 9 January 2001 | Millwall | 0 | - | 0 | Swindon Town (won 3-2 on pens.) | 2,394 | The New Den, London (aet) | 9 January 2001 | Peterborough United | 1 | - | 3 | Barnet | 1,891 | London Road, Peterborough | 9 January 2001 | Southend United | 3 | - | 1 | Cambridge United | 1,694 | Roots Hall, Southend-on-Sea | Quarter-finals | Northern section | 30 January 2001 | Bury | 0 | - | 3 | Chesterfield | 1,452 | Gigg Lane, Bury | 30 January 2001 | Lincoln City | 1 | - | 0 | Hartlepool United | 1,357 | Sincil Bank, Lincoln | 6 February 2001 | Port Vale | 4 | - | 0 | Darlington | 2,480 | Vale Park, Burslem | 7 February 2001 | Stoke City | 4 | - | 0 | Walsall | 5,624 | Britannia Stadium, Stoke | Southern section | 30 January 2001 | Barnet | 1 | - | 2 | Brentford | 1,438 | Underhill, London | 30 January 2001 | Southend United | 1 | - | 0 | Bristol Rovers | 2,192 | Roots Hall, Southend-on-Sea | 30 January 2001 | Swansea City | 1 | - | 0 | Reading | 2,516 | Vetch Field, Swansea | 30 January 2001 | Swindon Town | 2 | - | 1 | Wycombe Wanderers | 3,244 | County Ground, Swindon | Semi-Finals | Northern section | 13 February 2001 | Lincoln City | 4 | - | 1 | Chesterfield | 2,540 | Sincil Bank, Lincoln | 13 February 2001 | Port Vale (won in sudden death extra time by golden goal) | 2 | - | 1 | Stoke City | 11,323 | Britannia Stadium, Stoke (aet) | Southern section | 13 February 2001 | Southend United (won in sudden death extra time by golden goal) | 2 | - | 1 | Swindon Town | 3,337 | Roots Hall, Southend-on-Sea | 14 February 2001 | Swansea City | 2 | - | 3 | Brentford | 2,222 | Vetch Field, Swansea | Area Finals | Northern section: 1st leg | 13 March 2001 | Lincoln City | 0 | - | 2 | Port Vale | 4,813 | Sincil Bank, Lincoln | Southern section: 1st leg | 13 March 2001 | Southend United | 1 | - | 2 | Brentford | 5,055 | Roots Hall, Southend-on-Sea | Northern section: 2nd leg | 20 March 2001 | Port Vale | 0 | - | 0 | Lincoln City | 5,172 | Vale Park, Burslem | (Port Vale win 2-0 on aggregate) | Southern section: 2nd leg | 20 March 2001 | Brentford | 2 | - | 1 | Southend United | 6,579 | Griffin Park, London | (Brentford win 4-2 on aggregate) | National final | 22 April 2001 | Port Vale | 2 | - | 1 | Brentford | 25,654 | Millenium Stadium, Cardiff |
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